Confocal Microscopy - ZMB UZH

v10.0

11/27/2025 by Joana Raquel Delgado Martins

Printed Copies Are Uncontrolled

Wiki ID: 23

Author: Joana Raquel Delgado Martins

Release: v10.0 [Major]

Revision ID: 971

Revision Date: 11-27-25

Author: Dominik Hänni (and 4 other contributors)

How-to Guides

MD ImageXpress Confocal HT.ai (Irchel) - Datamover & file conversion

MD ImageXpress Confocal HT.ai (Irchel) - Datamover & file conversion

Nikon A1r - 2: acquisition in XY and Z

Nikon A1r - 2: acquisition in XY and Z

Nikon SD 2 (Irchel): Environmental control

Nikon SD 2 (Irchel): Environmental control

Nikon SD: How to connect Aria pump

Nikon SD: How to connect Aria pump

Zeiss LSM 980 Airyscan 2 - Part 2.2: How to acquire an image in "Airyscan" mode .

Zeiss LSM 980 Airyscan 2 - Part 2.2: How to acquire an image in "Airyscan" mode .

Support Questions

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Abberior INFINITY STED upright sample holder

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Evident Fluoview 4000 (Irchel) - 1: Start up

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Evident Fluoview 4000 (Irchel) - 2: Multichannel + Z-stack acquisition

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Evident Fluoview 4000 (Irchel) - Apply settings from an image

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Evident Fluoview 4000 (Irchel) - Shut Down

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Flatten Sample Holders Using Light Reflection Measurements

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Leica LAS X Navigator - Intro

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Leica SP8 (Lengg) - 1: Start up

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Leica SP8 (Lengg): Live Cell Imaging

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Leica SP8 inverse (Lengg): Shut down

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Leica SP8: Acquire an image in xy and z

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Leica LAS X Navigator - Intro

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Leica SP8 inverse (Gloriastrasse) - Part 1: Start-up

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Leica SP8 inverse (Gloriastrasse): Shut down

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Leica SP8: Acquire an image in xy and z

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Leica SP8s: Adjusting an objective with a Motorized Correction Ring

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Leica LAS X Navigator - Intro

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Leica SP8 inverse (IPZ) - Part 1: Start-up

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Leica SP8 inverse (IPZ): Shut down

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Leica SP8: Acquire an image in xy and z

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Leica LAS X Navigator - Intro

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Leica SP8 inverse (Irchel) - 1: Start-up

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Leica SP8 inverse (Irchel): Shut down

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Leica SP8: Acquire an image in xy and z

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Leica SP8 inverse (Schlieren) - Part 1: Start-up

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Leica SP8 inverse (Schlieren) - Part 2: Adjusting excitation and emission settings

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Leica SP8 inverse (Schlieren) - Part 3: Scan settings and z-stack recording

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Leica SP8 inverse (Schlieren) - Part 4: Data saving and instrument shut down

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Leica SP8 Inverse (Schlieren): Live Cell Imaging

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440nm pulsed laser on SP8 Falcon

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Leica LAS X Navigator - Intro

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Leica SP8 Falcon (Irchel) - Part 1: Start-up

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Leica SP8 Falcon (Irchel) - Part 2: Shut down

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Leica SP8 Falcon (Irchel): FLIM

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Leica SP8 Falcon and STED - Live cell imaging

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Leica SP8: Acquire an image in xy and z

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Leica LAS X Navigator - Intro

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Leica SP8 upright BL3 (Irchel) - 1: Start-up

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Leica SP8 upright BL3 (Irchel): Shut down

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Leica SP8: Acquire an image in xy and z

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Leica LAS X Navigator - Intro

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Leica Stellaris 1: Start-up

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Leica Stellaris 2: Acquire an image in xy and z

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Leica Stellaris 3 (Irchel) - Shut down

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Leica Stellaris 4 - Environmental control

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Leica Stellaris 5: Imaging optimization task (demo sample)

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Leica Stellaris TauSense modes

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Leica LAS X Navigator - Intro

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Leica Stellaris 5 - 2: Acquire an image in xy and z

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Leica Stellaris 5 inverse (USZ) - 1: Start-up

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Leica Stellaris 5 inverse (USZ) - 3: - Shut down

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Leica Stellaris 5 inverse (USZ) - How to get there

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Leica Stellaris 5: Imaging optimization task (demo sample)

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Leica Stellaris TauSense modes

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Leica LAS X Navigator - Intro

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Leica Stellaris 5 - 2: Acquire an image in xy and z

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Leica Stellaris 5 inverse (Bot) - 1: Start-up

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Leica Stellaris 5 inverse (Bot) - 3: - Shut down

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Leica Stellaris 5: Imaging optimization task (demo sample)

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Leica Stellaris TauSense modes

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Leica LAS X Navigator - Intro

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Leica Stellaris 5 - 2: Acquire an image in xy and z

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Leica Stellaris 5 upright (Bot) - 1: Start-up

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Leica Stellaris 5 upright (Bot) - Shut down

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Leica Stellaris 5: Imaging optimization task (demo sample)

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Leica Stellaris TauSense modes

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Leica LAS X Navigator - Intro

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Leica SP8 Falcon and STED - Live cell imaging

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Leica SP8 inverse STED 3X (Irchel) - 1: Start-up

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Leica SP8 inverse STED 3X (Irchel): Shut down

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Leica SP8: Acquire an image in xy and z

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Leica SP8s: Adjusting an objective with a Motorized Correction Ring

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Counting nuclei with MetaXpress

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MD ImageXpress Confocal HT.ai (Irchel) - 1: Multiplexing Introduction

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MD ImageXpress Confocal HT.ai (Irchel) - 1: Start up

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MD ImageXpress Confocal HT.ai (Irchel) - Datamover & file conversion

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MD ImageXpress Confocal HT.ai (Irchel): Acquiring Z stacks over time

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MD ImageXpress HT Data Analysis

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MD ImageXpress HT.ai: How to mark your data for deletion

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1. CLSM Nikon A1r+ - Start Up

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Nikon A1r - 2: acquisition in XY and Z

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Nikon A1r+ (Balgrist) - FRAP

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Nikon SD 1 (Irchel): Start Up

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Nikon SD 2 (Irchel): Environmental control

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Nikon SD 3 (Irchel) : Shutdown

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Nikon SD 4: Acquisition in XY and Z

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Nikon SD 5: Large image λXYZ

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Nikon SD 6: Multiwell plate imaging

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Nikon SD 7: Tissue imaging - 4 slide holder

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Nikon SD 8 - Setting up the DIC

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Nikon SD: How to connect Aria pump

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Nikon SD: Setting up a profile

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Nikon SD (Lengg) 1: Start Up

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Nikon SD 2 (Lengg): Environmental control

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Nikon SD 3 (Lengg) : Shutdown

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Nikon SD 4: Acquisition in XY and Z

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Nikon SD 5: Large image λXYZ

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Nikon SD 6: Multiwell plate imaging

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Nikon SD: Setting up a profile

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Olympus Spinning Disk - 1: Start up

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Olympus Spinning Disk - 2: Multichannel + Z-stack acquisition

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Olympus Spinning Disk - 3: Overviews + multi-positions + xyzt images

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Olympus Spinning Disk - Automatic sample detection using pretrained NN

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Olympus Spinning Disk - DIC images

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Olympus Spinning Disk - Image acquisition with cellSense for analysis in ScanR analysis software

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Olympus Spinning disk - Load new/different device settings

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Olympus Spinning Disk - Photomanipulation unit

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Olympus Spinning Disk - scanR Acquisition

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Olympus Spinning Disk - Shut Down

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Olympus Spinning Disk - Widefield mode

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Olympus Spinning Disk and ScanR - Datamover

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Perkin Elmer Operetta (Lengg) - 1: Start up

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Perkin Elmer Operetta (Lengg) -2: Acquire an Image in X-Y and Z

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Visitron Spinning Disk - 1: Start Up

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Visitron Spinning Disk - Acquire an Image in XY and Z

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Visitron Spinning Disk - Datamover

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Visitron Spinning Disk - Live Cell Set-up

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Visitron Spinning Disk - Shut down

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Zeiss LSM 980 Airyscan 2 - Part 1: Start-up

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Zeiss LSM 980 Airyscan 2 - Part 2.2: How to acquire an image in "Airyscan" mode .

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Zeiss LSM 980 Airyscan 2 / LSM 900 - Part 2.1: How to acquire an image in "LSM" mode

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Zeiss LSM 980 Airyscan 2 / LSM 900 - Part 3: Overviews, multi-positions and focusing strategies

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Data Mover ZEISS LSM 900 (Schlieren)

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XYZ Tracking macro

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Zeiss LSM 900 - Part 1: Start-up

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Zeiss LSM 980 Airyscan 2 / LSM 900 - Part 2.1: How to acquire an image in "LSM" mode

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Zeiss LSM 980 Airyscan 2 / LSM 900 - Part 3: Overviews, multi-positions and focusing strategies

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