Perkin Elmer Operetta (Lengg) - 1: Start up

Generally the system is always on, as this prevents condensation in the image formation path

Wait at least 30 sec. before you restart the Operetta CLS after a shutdown

Switch on the Operetta CLS (green button on the side)

The instrument is initialized (status light blinking green). As soon as the status light stops blinking (constantly green), the Operetta CLS is ready and can be used

Switch on the Harmony PC and log into Windows®

Notice It is recommended to let the instrument warm up for 1 hour before starting measurements. Otherwise drift as well as condensation artifacts can occur

Double-click the Harmony icon on the desktop

Log into Harmony. You will be given a user account after training

The Harmony Software has a bug where it indicates that it “isn’t connected to the device”

Override this connectivity error by pressing the “NEW” button under the C02

Afterwards you should see in the top-right corner that the software is connected when the plug symbol comes together

Main menu bar

Content area

Image control

Navigation

Global control

Settings

Messages

Results

The most important information is the plate type. Before loading a plate you have to select from the drop down menu the plate. If you plate is not on the list, you can add it with the instructions in the next step

For slides clean them and place them in the allocated holder, coverslip down

Clean the bottom of the plate with 100% EthOH and KimTech wipes

You might asked to adjust the objective collar after chooseing a plate, for this go to this guide

Press eject and the sample holder arm will come out

Put the plate in the holder

Press load and the plate will be taken inside the system. Make sure that there are no labels, sealing plastics around the plate and such

Go to "Settings"

"Define Plate Type"

Follow the wizard instructions

You can find the specifics of the plate with the manufacturer

The temperature needs to be adjusted at least 2hrs before the experiment. Otherwise z-drift could be experienced

Go to "Settings"

Then "TCO Settings"

Turn on the temperature and CO2 levels and adjust them as needed

Press OK

The gas connection is always on at 3 bar.