-
-
Start heating at least 30 min before you start your experiment. This allows the chamber and the microscope components to heat up and avoids major drift during your experiment.
-
Locate CO2 gas bottle close to the door
-
Open the main valve
-
Open the small valve
-
Locate the tubing in between Leica tables and open the blue valve for Stellaris.
-
Check water level in humidifier
-
If the water level is at minimum refill it from the stock bottle on the shelve above the microscope
-
-
-
Switch on the touchscreen of the okolab
-
Check the desired temperature in the box
-
If you need to change it. Click on the temperature and adjust +/-
-
Confirm Set
-
Once the temperature/level of CO2 is stabilized the yellow dot will turn green. For that you have to make sure, that environmental box is fully closed.
-
-
-
Find a live-cell chamber in the tower next to the microscope.
-
You can remove the magnetic lid and connect the tubing to the chamber.
-
Install the chamber on the microscpe
-
It is important that this tubing will around the galvo stage, otherwise it won't sits perfectly in the stage.
-
Block the other openings for dish/microscopic slide, if you don't use them. Otherwise the CO2 will escape from the chamber
-
Put the lid on top of the chamber
-
Close the environmental box around microscope to let temperature stabilize
-
