Nikon SD (Lengg) 1: Start Up

Start the computer below the table.

Start the microscope controller below the optical table

Start the spinning disk unit

Follow this guide if you need to turn on environmental control for live cell imaging.

Localize the water dispenser

Check if there is enough water in the bottle.

If not, find a bottle on the shelf above the microscope and re-fill the water dispenser.

If you see that there is almost no water left in the bottle, please let the ZMB staff know, e.g. via the feedback form! Same for immersion media, cleaning solutions

Pres Ctrl + Alt + Del and log in with your CORE credentials

Star the NIS-Elements SW

Star the software either in single or dual camera mode.

Open the top covers and lean back the condenser arm to access the stage.

Open the front cover

Make sure that the objective is in the lowest position. If you are not sure, press the escape button and reset the focus position by pressing it again and holding that for another 2-3 sec.

Place the insert into the stage. You can grab it by the red handles for easier handling.

Well plates directly fits into the stage. You do not have to insert any frame.

Mount your sample and move the stage that the objective is below the sample.

Choose the 4x Overview OC.

Go Live

Press the Autoscale button (Histogram with Play icon.) This will ensure that the Histogram will be scaled and you won't miss the sample even with a weak signal.

Alternatively, you can also select 4x objective and WF fluorescence channel for the overview if BF does not provide good contrast for sample localisation

Go up with the objective rotating the knob towards yourself.

The number of arrows indicates the speed of movement. (3 arrows = fastest)

You can change the speed with this button

Start with 3 or 2 arrows and go up carefully, until you will hear gentle beeping with which microscope indicates that it is near the focus (= it sense the reflection from a coverslip). Then change the speed to 1 arrow and find your focus.

Once you focused on the slide/sample, localize the Sample Navigation tab

Choose from the sample holder - if you cannot find the exact same one, choose from the most similar one.

Check different plane for different holders

Choose Service settings and Current Z position

Click on Preview

Microscope will acquire a tilescan, which you can use later for the navigation in the sample

If you are using a holder with a space for slide and two disches, select as a holder the Universal Holder - Full Area