Introduction
How to save your data and log off or shut down the Leica SP8 confocal laser scanning microscope located at the Schlieren campus, room WAD-12-K-105 once you are done..
Please find detailed information about the system setup here.
-
-
Go to "Open Projects" tab.
-
Save your data by either
-
right-clicking on the "Project"
-
using the "Save All" icon on top
-
or clicking the save sign behind the "Project"
-
Save your data on your core storage (network path: "\\files.core.uzh.ch\").
-
Please follow our instructions here on how to access your data.
-
-
-
You can save your settings and reuse them by loading them in your next imaging session. By default the following settings will be loaded: laser intensities, detector settings, averaging and accumulation settings.
-
Save your sequential scan settings if you want to reuse it (use "Home" directory on the server).
-
In your next imaging session you just need to activate "Sequential Scanning " and click "Load" in order to retrieve your scan settings.
-
Alternatively, you can also import a previously recorded LIF file, right click an image and select "Apply image settings".
-
-
-
Lower the objective and remove your sample.
-
Clean the immersion objective(s) you have used with the available "KIMTECH" wipes and 100% EtOH.
-
Always clean the lenses in concentric circles and from the center to the edge. Clean also the sides of the objective(s).
-
Please also take care that immersion media has been cleaned off from all the other microscope parts which got in contact with.
-
-
-
Check the booking system if there is another user scheduled within the next 2 hours.
-
-
-
Go to the "Configuration" tab and choose "Laser Config".
-
Does the booking system indicate another user within the next 2 hours?
-
If YES
-
keep the lasers ON or put on standby and reduce Argon output to 0%
-
close the "LAS X" software and Sign-Out of the Windows profile
-
If NO
-
switch OFF all lasers
-
close the "LAS X" software, shut-down the PC and follow the next step
-
-
-
Only follow this step if there is no booking within the next 2 hours.
-
Switch-OFF the fluorescence lamp
-
On the main switch board
-
turn the "Laser Emission" key to "Off-0"
-
Wait for 5 min to cool down the lasers
-
Finally switch OFF the "Laser Power", "Scanner Power" and "PC/Microscope" using the corresponding buttons
-
Team
