v2.0

by z mbstaff

Nikon SD 2: Setting up a profile

Written By: z mbstaff
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Difficulty

Easy

Steps

5

Time Required

00:05:00 - 00:10:00

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1

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In Progress

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Introduction

This guide will show you how to organise your profile in NIS-Elements.

  1. OC = Optical configurations, are like channels. In order to detect only signal from a specific dye, there has to be a specific filter/dichroic mirror, etc. in the optical path. And so that we don't have to set these elements manually in the SW, they are saved in so-called OCs.

    • OC = Optical configurations, are like channels. In order to detect only signal from a specific dye, there has to be a specific filter/dichroic mirror, etc. in the optical path. And so that we don't have to set these elements manually in the SW, they are saved in so-called OCs.

    • There is a set of pre-defined OCs available in your profile

    • This OCs are shared across users - and therefore the attributes like exposure time, bit depth, ROI, ... can be changed by anyone

    • If you want to have a private OCs and be sure you have consistent settings for your measurements we recommend to duplicate them in your folder

  2. If you want to create a sub-folder for your OCs: Click on the New Group and name the folder

    • If you want to create a sub-folder for your OCs:

    • Click on the New Group and name the folder

    • New groups will display below the shared OCs

    • We recommend to follow the structure and create a sub folder each for WF, SD and DIC (if needed)

  3. Right-click on the OCs you would like to use and duplicate Rename the OC - in this case we used the original name and initials from Zmb User For the naming you can also use what makes sense for you - e.g. excitation laser (488), or name of the dye you are using (GFP)

    • Right-click on the OCs you would like to use and duplicate

    • Rename the OC - in this case we used the original name and initials from Zmb User

    • For the naming you can also use what makes sense for you - e.g. excitation laser (488), or name of the dye you are using (GFP)

    • Drag and drop to your folder

    • With this, the OCs became your private ones - meaning, if you want to use consistent bitdepth or exposure time it will be saved in your OC

    • They will appear in your folder.

    • You can like that copy only those you are using for your experiment - in this case: WF DAPI and BF, and SD Cy7

  4. This step will help you to adjust the OC panel, so you can have an easy access to those that you use the most Localize the easy access OC panel Right-click on the panel and choose Select Visible Configurations

    • This step will help you to adjust the OC panel, so you can have an easy access to those that you use the most

    • Localize the easy access OC panel

    • Right-click on the panel and choose Select Visible Configurations

    • You can choose which of these OC you want to have displayed on the panel above for easy access.

  5. Rightclick on your OC and choose Edit You can choose from drag-and-drop menu from predefined colors Or you can specify color by wavelength and assigning by clicking on the arrow

    • Rightclick on your OC and choose Edit

    • You can choose from drag-and-drop menu from predefined colors

    • Or you can specify color by wavelength and assigning by clicking on the arrow

    • Once you are happy with new color, you can close the window and changes will take place

Finish Line

Author

z mbstaff

Member since: 05/06/2019

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