Difficulty
Easy
Steps
5
Time Required
00:05:00 - 00:10:00
- Nikon SD 2: Setting up a profile 5 steps
In Progress
This guide is currently being written. Reload periodically to see the latest changes.
Introduction
This guide will show you how to organise your profile in NIS-Elements.
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OC = Optical configurations, are like channels. In order to detect only signal from a specific dye, there has to be a specific filter/dichroic mirror, etc. in the optical path. And so that we don't have to set these elements manually in the SW, they are saved in so-called OCs.
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There is a set of pre-defined OCs available in your profile
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This OCs are shared across users - and therefore the attributes like exposure time, bit depth, ROI, ... can be changed by anyone
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If you want to have a private OCs and be sure you have consistent settings for your measurements we recommend to duplicate them in your folder
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If you want to create a sub-folder for your OCs:
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Click on the New Group and name the folder
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New groups will display below the shared OCs
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We recommend to follow the structure and create a sub folder each for WF, SD and DIC (if needed)
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Right-click on the OCs you would like to use and duplicate
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Rename the OC - in this case we used the original name and initials from Zmb User
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For the naming you can also use what makes sense for you - e.g. excitation laser (488), or name of the dye you are using (GFP)
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Drag and drop to your folder
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With this, the OCs became your private ones - meaning, if you want to use consistent bitdepth or exposure time it will be saved in your OC
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They will appear in your folder.
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You can like that copy only those you are using for your experiment - in this case: WF DAPI and BF, and SD Cy7
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This step will help you to adjust the OC panel, so you can have an easy access to those that you use the most
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Localize the easy access OC panel
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Right-click on the panel and choose Select Visible Configurations
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You can choose which of these OC you want to have displayed on the panel above for easy access.
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Rightclick on your OC and choose Edit
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You can choose from drag-and-drop menu from predefined colors
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Or you can specify color by wavelength and assigning by clicking on the arrow
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Once you are happy with new color, you can close the window and changes will take place
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