Introduction
This guide shows how you operate the incubator around the microscope stage.
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Open the gas bottles, or check if they are open.
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Open the switches for the CO2 and if you need for the N2 line.
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Open positition
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Turn on the gas and temperature controller. The three "on" switches are on the backside of the controllers.
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Switch on all three controllers.
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Adjust temperature.
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Set your gas concentrations
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Open the incubator box on the microscope.
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Check the water level of the bottle. It should be 60% filled for a weekend experiment.
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The dish has to be placed on top of the cell culture dish.
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Let the system equilibrate for 30 min, before you place your living sample and start your imaging.
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Do the steps in reverse, after you finish your experiment. Before you close the gas bottles check if someone else is using the gas supply for the GE Incell
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Team
