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Remove installed stage insert
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The stage incubator is located on the right inside the incubator box when not used.
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Install stage incubator on stage
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Incubator will be positioned on stage but does not slide in as the other stage inserts
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Only slide chambers or small round cell culture dishes can be loaded.
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The slide chamber is hold in place by a spring loaded tab.
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Load the sample by pressing the slide against the spring loaded tab and make sure it is positioned planar.
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The design of the Ibidi chamber slide allows only acquisition of 6 of the 8 wells. Lab-Tek slides work better in this sense.
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The stage incubator should be covered by the corresponding lid.
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Make sure you are using the right lid which you can find in a drawer box on the table.
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Ensure you have enough distance between lid and condensor.
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the condensor can be moved up/down by turning this wheel
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If there are no air bubbles visible in the water bottle ensure the CO2 valve is opened.
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The working distance of the 63x oil objective is to small for the stage incubator. If your cells are in focus and you reach the edge of chamber you will lift up the incubator by the objective. Please avoid this because it can break the objective!
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