TEM - TFS Glacios G2: How to restart the TEM server/computer

Perform initial checks of the microscope state before you start doing anything - make sure that:

The system vacuum is in order (all values in green range).

High Tension is on (at 200 kV).

There is no error messages in the status window.

The microscope is at LN2 temperatures (Autoloader tab).

In case the system is unresponsive (e.g. EPU is frozen) you might need to restart the microscope PC. To do so please:

Click on 'Stop All' (Software Launcher).

After a while, all the applications should close down and TEM server should stop.

Click on the 'Start' button and restart th PC.

After the system has rebooted login using the following credentials: USER: supervisor; PWD: Superuser#1

Once the Software Launcher has started: right click on the green 'Play' button and select 'Start Server and Applications'.

In case HT is off, click on High Tension button (so that it becomes yellow) and ramp it up to 200 kV.

Initialize autoloader.

In 'Alignements' tab load the correct file: APM_B00015992HT200.

Highlight all the settings and click the arrowhead to the left.

Then highlight them again and click 'Apply'.

Load the latest FEG registers by highlighting 'CURRENT' and clicking 'Set'.

In the Flu Cam viewer click on the 'CCD' button to display the green square that shows the area of the Falcon4i detector.

Launch EPU.

Load your settings by clicking 'Import' and selecting appropriate file.

Load Data Acquisition presets by clicking 'Set'. Make sure that the beam covers the detector. Then click 'Measure'.

After a moment, the current electron dose in e/pix/s will show up. Make sure you stay in the green range. The optimal dose is between 5 and 10 e/pix/s.