Skip to main content

v7.0

by z mbstaff

Introduction

How to start the software and mount the sample using the airlock of the Zeiss Gemini 450 SEM at the Center for Microscopy and Image Analysis, University of Zurich.

  1. Log in to the computer with your ZMB credentials. Wait until the feedback form loads! Start SmartSEM software (icon on desktop).
    • Log in to the computer with your ZMB credentials.

    • Wait until the feedback form loads!

    • Start SmartSEM software (icon on desktop).

    • Enter username and password: user, user.

    • Mount your samples on the sample holder.

    • Always wear gloves!

  2. Press "Vent" on the airlock panel. Open the airlock and insert the sample holder. Attach the manipulator rod to the sample holder. Hold the holder with one hand and screw in the rod with the other hand.
    • Press "Vent" on the airlock panel.

    • Open the airlock and insert the sample holder. Attach the manipulator rod to the sample holder.

    • Hold the holder with one hand and screw in the rod with the other hand.

    • Acquire a preview image by pressing the camera button on the airlock or in the software. Wait until the image appears in teh SmartSEM software.

  3. Close the airlock and press "Exchange" in the control panel. Wait until the transfer LED is on and not flashing anymore. Use the white piece at the far end of the rod to slowly insert the sample holder into the microscope.
    • Close the airlock and press "Exchange" in the control panel.

    • Wait until the transfer LED is on and not flashing anymore.

    • Use the white piece at the far end of the rod to slowly insert the sample holder into the microscope.

    • Unscrew the rod and use again the white piece at the far end to retract the rod to the lock position.

    • Press Resume in the control panel.

  4. Select "EHT" and set it to "EHT on".
    • Select "EHT" and set it to "EHT on".

    • Use image navigation to move the sample below the lens.

Finish Line

One other person completed this guide.

José Maria Mateos Melero

Member since: 04/28/2019

1,080 Reputation

12 Guides authored

Team

ZMB Staff Member of ZMB Staff

11 Members

139 Guides authored

0 Comments

Add Comment

View Statistics:

Past 24 Hours: 1

Past 7 Days: 6

Past 30 Days: 36

All Time: 1,398