Nikon Ti2-E (Irchel) - Start up and overview

control unit

camera

microscope computer

turn the microscope stand on

turn the camera on

turn the microscope control unit on

turn the PC on (check if the screen is also switched on)

Log in to windows with your zmb credentials

Open Nikon "NIS-Elements"

Choose "Fusion"

Open devices

Enable mouse joystick Z in live

Activate mouse XY, while live acquisition

With this seeting active you can use the mouse in the software to move the stage.

Choose one of the preset optical configurations, that fits to your fluorophore.

Choose illumination wavelength (when activated - highlighted blue)

Choose excitiation filter

Choose emission filter

Choose 2x objective for overview acquisition

Click on "Live"

Click here to display over and undersaturated pixels.

oversaturated pixels in blue. The highlight colour can be changed.

Adjust the look up table

Use the mouse wheel in the live window to focus the sample.

Adjust exposure time to your imaging requirements

Go to ND Acquisition window

Setup "Large Image"

Setup Lambda

Click on "Run now" to start imaging an overview

Right click into overview image

"Use as preview in XYZ overview"

Go "Live"

Adjust look up table

bring sample to focus (use mouse wheel)

Right click in XYZ Overview area

Choose "Scan 3x3"

Use overview for navigation