Skip to main content

v3.0

by z mbstaff

Introduction

This guide shows how you operate the incubator around the microscope stage.

  1. Open the gas bottles, or check if they are open.
    • Open the gas bottles, or check if they are open.

    • Open the switches for the CO2 and if you need for the N2 line.

    • Open positition

  2. Turn on the gas and temperature controller. The three "on" switches are on the backside of the controllers. Switch on all three controllers.
    • Turn on the gas and temperature controller. The three "on" switches are on the backside of the controllers.

    • Switch on all three controllers.

    • Adjust temperature.

    • Set your gas concentrations

  3. Open the incubator box on the microscope. Check the water level of the bottle. It should be 60% filled for a weekend experiment.
    • Open the incubator box on the microscope.

    • Check the water level of the bottle. It should be 60% filled for a weekend experiment.

    • The dish has to be placed on top of the cell culture dish.

    • Let the system equilibrate for 30 min, before you place your living sample and start your imaging.

    • Do the steps in reverse, after you finish your experiment. Before you close the gas bottles check if someone else is using the gas supply for the GE Incell

Finish Line

z mbstaff

Member since: 05/06/2019

3,323 Reputation

44 Guides authored

Team

Group Lopes Member of Group Lopes

6 Members

87 Guides authored

0 Comments

Add Comment

View Statistics:

Past 24 Hours: 0

Past 7 Days: 3

Past 30 Days: 6

All Time: 61