Introduction
This guide shows where to find the image acquisition and image data information from raw data. These two components are part of the complete metadata of a study . For further reading REMBI: Recommended Metadata for biological images-enabling reuse of microscopy data in Biology
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Open FiJI
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In Tab File, select Import and Bio-format
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Select the image to open.
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A new window will appear.
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In the metadata viewing part. Check to activate Display metadata and Display metadata OME-XML metadata
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Two windows with the metadata information will appear together with the image
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Open the file on LAS application
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Right-click on the image you want to know its metadata
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Select Properties
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A new window will appear showing the metadata.
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Leica data format is .lif
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Open the data with ZEN sofware
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Click on the info tab to see the metadata values
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Zeiss data format is .czi
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Open the data with MAPS and on the lower-left corner you can read some metadata parameters.
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MesoSPIM saves the data in h5 format and the metadata can be found in an associated .txt file. Open it with any text editor.
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MesoSPIM data format is .h5
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Open the data with Abberior Lightbox software.
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Click on the three points icon on the right side of each image.
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Select metadata.
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A new window will appear with the metadata information.
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Abberior data format is .obf
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Open the data with CellSens Dimension Desktop.
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Right-Click on the image and from the appearing window, select properties.
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A new window with the metadata will appear.
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This metadata can be exported as .txt file by clicking on the "save properties as txt file" icon.
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Evident data format is .oir
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Open the file with VisiView software.
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In the Main menu select: "File" and from the drop-down menu select: "File Info".
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A window will appear with the image metadata.
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Data can be saved in Visitron data format .stk
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or in ome.tf2 Format OME Big-Tiff (recommended)
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