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Loging to microscope computer using SEMUser as user and password.
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Start "SEM Operation" software
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Press "Specimen exchange".
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Activate "Specimen exchange" in the popup menu.
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Activate "Move stage to exchange position" and confirm with "Yes (Y)"
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Open door of instrument using the handle on the right side.
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Remove sample holder as indicated on the screen.
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Press "OK"
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Use regular stubs for Zeiss instruments.
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Mount on regular Zeiss holder and attach with the screws.
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Clip Zeiss holder into Jeol carrier. Only one orientation possible.
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Holder assembly.
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Measure height of the whole assembly with dedicated tool. Top of sample to bottom distance.
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It is important to measure correctly.
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You must create a new file or open an existing one.
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For creating, press "Create..."
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Select proper holder (bright green square: 51 mm).
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Click "Add"
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Insert name of choice and click "Add" in the popup window.
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Adjust height measured in the previous step using the slider.
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Make sure you select the correct height. If value is too low by mistake, the backscatter detector may be damaged.
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Press "OK" and confirm.
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Select observation conditions.
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Press "OK"
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Insert sample holder into chamber as indicated on the screen.
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Close door.
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Press "EVAC" and confirm.
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Press "Reacquire Photo"
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Stage will move to camera position and acquire an image.
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Pres "OK"
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Select appropriate stage movement. In general "Go to specified area and set WD to 10.0 mm".
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Select the region of interest by clicking on desired location.
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Press "Start Observation".
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Observe the stage movement using the chamber scope.
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You may interrupt the stage movement if it moves too close towards the lens: purple line (only happens if height not measured properly).
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Start imaging by clicking "Observation".
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Electron beam will be turned on and column valve opens.
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Press "Fast" to start scanning/unfreeze.
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EDX system is always on and measures spectra on the go.
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