Introduction
On the MesoSPIM, the shutters can be in manual mode after initializing the system. This leads to no laser emission when imaging or to a situation where both illumination paths are emitting light at the same time. In this guide we explain you how to fix those issues.
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Check that the lasers are on and that the "Emission" LED is also on.
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Press "Live" in the MesoSPIM control software and check if the LED of the shutter is "on" in the corresponding illumination path (left or right).
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If the LED is "off" then the shutter is in manual mode and has to be changed to external trigger mode:
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1. Press once "ENABLE" to leave in “on” position
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2. Press and hold "ENABLE" for few seconds until the LED blinks and switch it off.
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Done.
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Press live in the MesoSPIM control software and check if the LEDs of both shutters are "on" (left and right).
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If both LEDs are on, then the shutters are in manual mode and have to be changed to external trigger mode.
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Press and hold "ENABLE" for few seconds until the LED blinks and switches off. Do this for both sides.
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Done.
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