Introduction
In this guide you will learn how to start-up and mount a sample on the ZMB MesoSPIM lightsheet microscope.
For a full documentation about the microscope please, visit the project webpage: http://MesoSPIM.org
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Switch on computer located below the table.
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Log-in to instrument computer with your ZMB credentials.
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At the back of the mesoSPIM. Turn-on the two power supplies for the galvo systems.
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Turn the laser keys to "ON" position.
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Open the light insulation box back door for accessing the camera and turn it on.
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Turn-on the two galvo mirrors.
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Double click on the "MesoSPIM Control" icon. A window will appear to select the ETL parameters.
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Select your personal ETL configuration file which has been created during your microscope introduction.
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The microscope control, camera and acquisition manager windows will appear. Select the "Movement" menu and proceed with the sample loading.
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Take the immersion cuvette according to your sample from the chemical hood.
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Insert the sample holder with the two two balls to the back.
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In the software use the arrow button to bring the sample down into the immersion chamber.
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Close the doors of the light insulation box.
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