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On the main menu, select "Advanced modules" and from the drop-down menu, select "Fuse Views"
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The package's window will open, and you will be able to modify the fusing parameters on the top panel.
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At the bottom panel you will see updates on the status of the fusing, or if something is not working.
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Indicate the folder that contains your experiment. This folder should contain all positions and stacks you have imaged. The software will later ask which position(s) to fuse.
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Indicate the "Crop size". This is some degree of offset between the two views, as well as limiting the fusing to your areas of interest.
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Indicate the group of "Settings" that is to be fused.
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Indicate the channel to fuse with the name of the excitation wavelength. Options are: "488", "561", "638", and "Transmitted".
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You will get an update on whether you've set up things correctly. If so then you can continue.
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To begin the fusing you click on "Start"
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As you click start the window will open where you will select which position to sure. Select it.
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Almost done!
Finish Line