Introduction
In this guide of the Center for Microscopy and Image Analysis we show how to shut down the Leica Stellaris 5 confocal laser scanning microscope located at the UZH, Zollikerstrasse 107, 8008 Zürich.
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Go to the "Open projects" tab.
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Right-click on your data file and give it a meaningful name.
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Click on the disk button to save the data. Alternatively right click on the specific "project" and choose "Save as..." from the drop down menu.
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Save your (sequential) scan settings if applicable.
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In order to retrieve your saved scan settings in your next imaging session click "Load".
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Lower the objective and remove your sample.
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Clean the immersion objective(s) you have used with the available "KIMTECH" wipes and 100% EtOH.
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Switch to the 5x dry objective within the software.
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Go to "Configuration" and choose "Laser Config".
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If YES:
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keep the lasers "ON" or put on "standby",
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close the "LAS X" software and Sign out of your Windows profile.
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If NO :
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switch OFF all lasers ,
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close the "LAS X" software, shut-down the PC and follow the next step.
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Turn off the Laser key (control unit next to the microscope) and switch OFF the "Laser" and "Power " switches.
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