Introduction
In this guide of the Center for Microscopy and Image Analysis we show how to shut down the Leica SP8 inverse confocal laser scanning microscope located at Y42-H-81.
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Go to "Open Projects" tab.
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Save your data
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by either right-clicking on the "Project",
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using the "Save All" icon on top,
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or clicking the save sign behind the "Project".
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Please save your data on your core storage (network path: "\\files.core.uzh.ch\").
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Please follow our instructions here on how to access your data.
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You can save your settings and reuse them by loading them in your next imaging session. By default the following settings will be loaded: laser intensities, detector settings, averaging and accumulation settings.
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Save your sequential scan settings if applicable.
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In your next imaging session you just need to activate "Seq" and click "Load" in order to retrieve your scan settings.
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To save only single channel settings:
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Click the "Save-Sign" and choose an appropriate name for saving.
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Click "Ok".
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You can now find your single channel setting under "User Settings".
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Lower the objectives and remove your sample.
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Clean the immersion objective(s) you have used with the available "KIMTECH" wipes and 100% EtOH.
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Always clean the lenses in concentric circles and from the center to the edge. Clean also the sides of the objective(s).
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Please also take care that immersion media has been cleaned off of all the other microscope parts which got in touch with.
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Switch to the 10x dry objective.
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Only follow this step if environmental control was applied during your experiment.
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37°C and 5% CO2 are set as a standard. If you have changed it during your experiment, please set it back to the standard values.
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Switch off the heating cube (underneath microscope table).
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Switch off the brick (CO2 control).
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Close the dedicated valves of the gas supplies.
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Go to "Configuration" and choose "Laser Config".
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If applicable, lower the power to 0% for the STED lasers.
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Switch OFF all lasers.
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If STED was used, turn OFF the Laser Emission Keys of the 592nm and 660nm STED lasers.
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Check in the booking system if there is another booking within the next 2 hours.
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If YES, close the "LAS X" software and Sign-Out.
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If NO, close the "LAS X" software, shut-down the PC and follow the next step.
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Only follow this step if there is no booking within the next 2 hours.
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Switch-OFF the fluorescence lamp.
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On the main switch board
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turn the "Laser Emission" key to "Off-0",
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and switch OFF the "Scanner Power", "Laser Power" and "PC/Microscope".
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Take care the PC has been fully shut-down before.
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