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v4.0

by Karin Seubert

Introduction

In this guide of the Center for Microscopy and Image Analysis we show how to shut down the Leica SP8 inverse confocal laser scanning microscope located at the Institute of Medical Microbiology.

Please find detailed information about the system setup here.

  1. Go to "Open Projects" tab.
    • Go to "Open Projects" tab.

    • Save your data

    • by either right-clicking on the "Project",

    • using the "Save All" icon on top,

    • or clicking the save sign behind the "Project".

    • Please save your data on your core storage (network path: "\\files.core.uzh.ch\").

    • Please follow our instructions here on how to access your data.

  2. You can save your settings and reuse them by loading them in your next imaging session. By default the following settings will be loaded: laser intensities, detector settings, averaging and accumulation settings. Save your sequential scan settings if applicable. In your next imaging session you just need to activate "Seq" and click "Load" in order to retrieve your scan settings.
    • You can save your settings and reuse them by loading them in your next imaging session. By default the following settings will be loaded: laser intensities, detector settings, averaging and accumulation settings.

    • Save your sequential scan settings if applicable.

    • In your next imaging session you just need to activate "Seq" and click "Load" in order to retrieve your scan settings.

    • To save only single channel settings:

    • Click the "Save-Sign" and choose an appropriate name for saving.

    • Click "Ok".

    • You can now find your single channel setting under "User Settings".

  3. Lower the objectives and remove your sample. Clean the immersion objective(s) you have used with the available "KIMTECH" wipes and 100% EtOH.
    • Lower the objectives and remove your sample.

    • Clean the immersion objective(s) you have used with the available "KIMTECH" wipes and 100% EtOH.

    • Always clean the lenses in concentric circles and from the center to the edge. Clean also the sides of the objective(s).

    • Please also take care that immersion media has been cleaned off of all the other microscope parts which got in touch with.

  4. Switch to the 10x dry objective.
    • Switch to the 10x dry objective.

    • Only follow this step if environmental control was applied during your experiment.

    • 37°C and 5% CO2 are set as a standard. If you have changed it during your experiment, please set it back to the standard values.

    • Switch off the heating cube.

    • Switch off the brick (CO2 control).

    • Close the dedicated valves of the gas and air supplies.

  5. Go to "Configuration" and choose "Laser Config".
    • Go to "Configuration" and choose "Laser Config".

    • Check in the booking system if there is another booking within the next 2 hours.

    • If YES

    • keep the lasers ON or put on standby,

    • close the "LAS X" software and Sign-Out.

    • If NO

    • switch OFF all lasers ,

    • close the "LAS X" software, shut-down the PC and follow the next step.

  6. Only follow this step if there is no booking within the next 2 hours. Switch-OFF the fluorescence lamp. On the main switch board
    • Only follow this step if there is no booking within the next 2 hours.

    • Switch-OFF the fluorescence lamp.

    • On the main switch board

    • turn the "Laser Emission" key to "Off-0".

    • Wait for 5 min to cool down the lasers.

    • Finally switch OFF the "Scanner Power", "Laser Power" and "PC/Microscope".

    • Take care the PC has been fully shut-down before.

Finish Line

Jana Döhner

Member since: 04/10/2019

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