File Maker - Setting up a new experiment for LM Imaging service

Open the Experimental Procedures / Project Experiment Database.

Create a new Experiment.

Add basic info.

Select both that it is serviced based and that a a bill needs to be issued.

Add to whom is the serviced going to be billed.

Add the image modality that will be used.

(ec) Experimental condition stands for the sample - in this case brain fixed with 4% PFA or 2.5% GA

(sa) Sample stands for the slide containing sections from this sample

(sc) sample carrier defines the image acquired for each section, ROI, well etc.

9863-ec-01-sa-01-sc-01 would be:

9863 - project

ec - 01 - brain 4% PFA

sa - 01 - slide 1 containing 1 or more sections/ROI

sc - 01 - image acquired from section/ROI 1

Your images will need to be named according to structure you have just provided for your experiment.

Click on the copy button and past it in the description of your sample carrier.

If the sample was prepared at the ZMB and will be kept, please create a new entry in the storage button

In this example an image acquired in the well 1 b would have the naming

9863-ec-01-sa-01-sc-01

sa -

sc -

You will use this file names to label your images.

Add the protocol used to prepare the sample. or is sample already provided a small description eg . Histology slide Slides were provided by the Anatomy department using different Histology preparation techniques.

Add all the Imaging Job description that would be necessary to reproduce your results.

Access the folder where your data will be saved.

Example images - keep screenshots or small files to quickly have an idea of what is contained here (available after archiving).

Processed Data -

Protocol Notes - all extra information, or documents that you couldn't add in the experiment entry. eg. protocols from users or presentations.

also save quotes and invoices here.

Raw Data - keep all your raw data here

Temporary Data - will be deleted after archiving. can be useful to have for testing or support processing while working on the project.

If you need to track prep times or scanning times you can use the tab next to experimental conditions.

Keep in mind that it should be clearly stated how much time was spent per slide to aid billing.

Additional information should be added to the next item.

To add even more details please click here.

Here you can log any issues that you had during preparation for imaging and how were they solved. Consider information that might be useful or a future service or to justify billing.

You are ready to go! Happy imaging!!!