Introduction
How to set up a new experiment in File maker for LM Imaging service.
For emails, meeting logs please add an entry in One Note with the same project number.
ZMB Notebook > Service in sample preparation and image processing > Light microsocopy
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Open the Experimental Procedures / Project Experiment Database.
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Create a new Experiment.
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Add basic info.
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Select both that it is serviced based and that a a bill needs to be issued.
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Add to whom is the serviced going to be billed.
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Add the image modality that will be used.
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(ec) Experimental condition stands for the sample - in this case brain fixed with 4% PFA or 2.5% GA
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(sa) Sample stands for the slide containing sections from this sample
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(sc) sample carrier defines the image acquired for each section, ROI, well etc.
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9863-ec-01-sa-01-sc-01 would be:
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9863 - project
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ec - 01 - brain 4% PFA
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sa - 01 - slide 1 containing 1 or more sections/ROI
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sc - 01 - image acquired from section/ROI 1
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Your images will need to be named according to structure you have just provided for your experiment.
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Click on the copy button and past it in the description of your sample carrier.
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If the sample was prepared at the ZMB and will be kept, please create a new entry in the storage button
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In this example an image acquired in the well 1 b would have the naming
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9863-ec-01-sa-01-sc-01
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You will use this file names to label your images.
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Add the protocol used to prepare the sample. or is sample already provided a small description eg . Histology slide Slides were provided by the Anatomy department using different Histology preparation techniques.
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Add all the Imaging Job description that would be necessary to reproduce your results.
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Access the folder where your data will be saved.
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Example images - keep screenshots or small files to quickly have an idea of what is contained here (available after archiving).
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Processed Data -
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Protocol Notes - all extra information, or documents that you couldn't add in the experiment entry. eg. protocols from users or presentations.
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also save quotes and invoices here.
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Raw Data - keep all your raw data here
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Temporary Data - will be deleted after archiving. can be useful to have for testing or support processing while working on the project.
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If you need to track prep times or scanning times you can use the tab next to experimental conditions.
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Keep in mind that it should be clearly stated how much time was spent per slide to aid billing.
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Additional information should be added to the next item.
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To add even more details please click here.
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Here you can log any issues that you had during preparation for imaging and how were they solved. Consider information that might be useful or a future service or to justify billing.
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You are ready to go! Happy imaging!!!
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